Antisense downregulation of SARS-CoV gene expression in Vero E6 cells.
Identifieur interne : 004927 ( Main/Exploration ); précédent : 004926; suivant : 004928Antisense downregulation of SARS-CoV gene expression in Vero E6 cells.
Auteurs : Yi Shi [République populaire de Chine] ; Haifeng Luo ; Jie Jia ; Jie Xiong ; Dehua Yang ; Bing Huang ; Youxin JinSource :
- The journal of gene medicine [ 1099-498X ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Microscopie confocale, Oligonucléotides antisens (), Oligonucléotides antisens (génétique), Oligonucléotides antisens (métabolisme), Oligonucléotides antisens (pharmacologie), Plasmides, Protéines de fusion recombinantes (métabolisme), Protéines luminescentes (génétique), Protéines à fluorescence verte (métabolisme), RT-PCR, Relation dose-effet des médicaments, Régulation de l'expression des gènes viraux, Régulation négative, Virus du SRAS (génétique), Virus du SRAS (métabolisme).
- MESH :
- génétique : Oligonucléotides antisens, Protéines luminescentes, Virus du SRAS.
- métabolisme : Oligonucléotides antisens, Protéines de fusion recombinantes, Protéines à fluorescence verte, Virus du SRAS.
- pharmacologie : Oligonucléotides antisens.
- Animaux, Cellules Vero, Microscopie confocale, Oligonucléotides antisens, Plasmides, RT-PCR, Relation dose-effet des médicaments, Régulation de l'expression des gènes viraux, Régulation négative.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Dose-Response Relationship, Drug, Down-Regulation, Gene Expression Regulation, Viral, Green Fluorescent Proteins (metabolism), Luminescent Proteins (genetics), Microscopy, Confocal, Oligonucleotides, Antisense (chemistry), Oligonucleotides, Antisense (genetics), Oligonucleotides, Antisense (metabolism), Oligonucleotides, Antisense (pharmacology), Plasmids, Recombinant Fusion Proteins (metabolism), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (metabolism), Vero Cells.
- MESH :
- chemical , chemistry : Oligonucleotides, Antisense.
- chemical , genetics : Luminescent Proteins, Oligonucleotides, Antisense.
- chemical , metabolism : Green Fluorescent Proteins, Oligonucleotides, Antisense, Recombinant Fusion Proteins.
- chemical , pharmacology : Oligonucleotides, Antisense.
- genetics : SARS Virus.
- metabolism : SARS Virus.
- Animals, Chlorocebus aethiops, Dose-Response Relationship, Drug, Down-Regulation, Gene Expression Regulation, Viral, Microscopy, Confocal, Plasmids, Reverse Transcriptase Polymerase Chain Reaction, Vero Cells.
Abstract
Severe acute respiratory syndrome (SARS) is caused by a novel coronavirus (SARS-CoV). It is an enveloped, single-stranded, plus-sense RNA virus with a genome of approximately 30 kb. The structural proteins E, M and N of SARS-CoV play important roles during host cell entry and viral morphogenesis and release. Therefore, we have studied whether expression of these structural proteins can be down-regulated using an antisense technique.
DOI: 10.1002/jgm.640
PubMed: 15543523
Affiliations:
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Le document en format XML
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des gènes viraux</term><term>Régulation négative</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is caused by a novel coronavirus (SARS-CoV). It is an enveloped, single-stranded, plus-sense RNA virus with a genome of approximately 30 kb. The structural proteins E, M and N of SARS-CoV play important roles during host cell entry and viral morphogenesis and release. Therefore, we have studied whether expression of these structural proteins can be down-regulated using an antisense technique.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Huang, Bing" sort="Huang, Bing" uniqKey="Huang B" first="Bing" last="Huang">Bing Huang</name><name sortKey="Jia, Jie" sort="Jia, Jie" uniqKey="Jia J" first="Jie" last="Jia">Jie Jia</name><name sortKey="Jin, Youxin" sort="Jin, Youxin" uniqKey="Jin Y" first="Youxin" last="Jin">Youxin Jin</name><name sortKey="Luo, Haifeng" sort="Luo, Haifeng" uniqKey="Luo H" first="Haifeng" last="Luo">Haifeng Luo</name><name sortKey="Xiong, Jie" sort="Xiong, Jie" uniqKey="Xiong J" first="Jie" last="Xiong">Jie Xiong</name><name sortKey="Yang, Dehua" sort="Yang, Dehua" uniqKey="Yang D" first="Dehua" last="Yang">Dehua Yang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Shi, Yi" sort="Shi, Yi" uniqKey="Shi Y" first="Yi" last="Shi">Yi Shi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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